Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5850330 | Food and Chemical Toxicology | 2013 | 7 Pages |
Abstract
In this pilot survey the levels of various mycotoxin biomarkers were determined in third trimester pregnant women from eastern Croatia. First void urine samples were collected and analysed using a “dilute and shoot” LC-ESI-MS/MS multi biomarker method. Deoxynivalenol (DON) and its metabolites: deoxynivalenol-15-glucuronide and deoxynivalenol-3-glucuronide were detected in 97.5% of the studied samples, partly at exceptionally high levels, while ochratoxin A was found in 10% of the samples. DON exposure was primarily reflected by the presence of deoxynivalenol-15-glucuronide with a mean concentration of 120 μg Lâ1, while free DON was detected with a mean concentration of 18.3 μg Lâ1. Several highly contaminated urine samples contained a third DON conjugate, tentatively identified as deoxynivalenol-7-glucuronide by MS/MS scans. The levels of urinary DON and its metabolites measured in this study are the highest ever reported, and 48% of subjects were estimated to exceed the provisional maximum tolerable daily intake (1 μg kgâ1 b.w.).
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Authors
Bojan Å arkanj, Benedikt Warth, Silvio Uhlig, Wilfred A. Abia, Michael Sulyok, Tomislav Klapec, Rudolf Krska, Ines Banjari,