Suppressive effects of Indigofera suffruticosa Mill extracts on lipopolysaccharide-induced inflammatory responses in murine RAW 264.7 macrophages

Article ID	Journal	Published Year	Pages	File Type
5851542	Food and Chemical Toxicology	2013	8 Pages	PDF

Abstract

âº To study the anti-inflammatory effect of I. suffruticosa in LPS-induced macrophages. âº I. suffruticosa extracts decreased LPS-induced inflammation and NF-ÎºB activation. âº I. suffruticosa extracts induced HO-1 expression through ERK/Nrf2 pathway. âº HO-1 siRNA partly reversed the anti-inflammatory effects of I. suffruticosa extracts.

Keywords

HO-1 High performance liquid chromatography/mass spectrometry Indigofera suffruticosa 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide SEAP Heme oxygenase-1 IL-1β PMSF iNOS NF-kB GAPDH Nrf2 NF-κB LPS NTC DMSO ERK1/2 HPLC/MS MTT Small interfering RNA siRNA Electrophoretic mobility shift assay Secretory alkaline phosphatase inflammation Interleukin-1β tumor necrosis factor-α Dimethyl sulfoxide Syringaldehyde inducible nitric oxide synthase EMSA یا electrophoretic mobility shift assay nuclear factor E2-related factor 2 antioxidant response element TNF-α Nuclear factor-kappa B phenylmethylsulfonyl fluoride lipopolysaccharide carbon monoxide Nitric oxide ARE polymerase chain reaction PCR Syr extracellular signal-regulated kinase 1/2 glyceraldehyde-3-phosphate dehydrogenase