Ferric citrate CYP2E1-independently promotes alcohol-induced apoptosis in HepG2 cells via oxidative/nitrative stress which is attenuated by pretreatment with baicalin

Article ID	Journal	Published Year	Pages	File Type
5852241	Food and Chemical Toxicology	2012	9 Pages	PDF

Abstract

âº Iron CYP2E1-independently promotes alcohol-induced oxidative/nitrative stress. âº Baicalin pretreatment prevents HepG2 cells from death induced by iron and alcohol. âº The main mechanism of cell protection by baicalin is through iron chelation.

Keywords

Deferoxamine CYP2E1 Baicalin ECL PAGE RNS iNOS GAPDH DFO 4-HNE SDS 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide 4-hydroxy-2-nonenal MTT ROS ALD Iron Ethanol polyacrylamide gel electrophoresis alcohol liver disease alcoholic liver disease Oxidative stress sodium dodecyl sulfate inducible nitric oxide synthase cytochrome P450 2E1 Ferric citrate Nitric oxide Glyceraldehyde phosphate dehydrogenase reactive nitrogen species Reactive oxygen species