| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5852651 | Food and Chemical Toxicology | 2012 | 5 Pages |
Cultivated Coptis chinensis inflorescence has been highly valued in Chinese tea production for many years. The main alkaloid compounds in C. chinensis inflorescence ethanolic extracts (CE) were identified by high-performance liquid chromatography-mass spectrometry. The detected compounds included jatrorrhizine (4.87Â mg/g), coptisine (17.18Â mg/g), palmatine (3.32Â mg/g), and berberine (31.81Â mg/g), as well as columbamine and epiberberine (tentatively identified). CE protective activity against ultraviolet-B (UVB)-induced phototoxicity in a mitochondria model was determined by measuring thiobarbituric acid-reactive substrates, lipid hydroperoxide, conjugated diene, 4-hydroxynonenal, and glutathione. The results showed that CE excellently inhibited UVB-induced lipid peroxidation and glutathione reduction in vitro. This photoprotective effect of CE may be caused by the presence of the abovementioned alkaloid compounds and phenolic compounds that enhances CE antioxidant activity. Therefore, CE possesses potent photoprotective property that may find valuable applications in food industries and in anti-phototoxicity formulations.
⺠The main alkaloid compounds in Coptis chinensis inflorescence ethanolic extracts (CE) were identified by HPLC-MS. ⺠In a UVB-induced mitochondria model, CE has strong protective activity against phototoxicity. ⺠CE showed excellently protective activity of UVB-induced lipid peroxidation and glutathione reduction. ⺠Research on this function of C. chinensis inflorescence may broaden its applications in the medical and food industry.
