Anti-inflammatory activity of Korean thistle Cirsium maackii and its major flavonoid, luteolin 5-O-glucoside

Article ID	Journal	Published Year	Pages	File Type
5852906	Food and Chemical Toxicology	2012	9 Pages	PDF

Abstract

âº Luteolin 5-O-glucoside from C. mackii whole plants possess anti-inflammatory activity. âº The presence of a 5-O-glucosyl group in luteolin is required to reduce the cytotoxicity. âº Luteolin 5-O-glucoside inhibited LPS-induced NO production. âº It also inhibited t-BHP-induced ROS generation. âº It suppressed the expression of iNOS and COX-2 in macrophages.

Keywords

DTPA DCFH iNOS DCFH-DA NOS COX-2 HRP TLC RNS Dcf AMT PVDF DPPH DMSO-d6 tert-butylhydroperoxide LPS FBS DMEM DHR 123 1,1-diphenyl-2-picrylhydrazyl 2′,7′-dichlorodihydrofluorescein 2′,7′-dichlorodihydrofluorescein diacetate 2′,7′-dichlorofluorescein 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid MTT ROS t-BHP EDTA Ethylenediaminetetraacetic acid Trolox Diethylenetriaminepentaacetic acid dihydrorhodamine 123 fetal bovine serum inducible nitric oxide synthase Cyclooxygenase-2 Anti-inflammatory lipopolysaccharide Dulbecco’s modified eagle’s medium Nitric oxide nitric oxide synthase Horseradish peroxidase prostaglandin Polyvinylidene fluoride thin layer chromatography HPLC reactive nitrogen species Reactive oxygen species