Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5853878 | Food and Chemical Toxicology | 2011 | 6 Pages |
Abstract
The modifying effect of chemically modified chrysin on formation of preneoplastic foci induced by N-nitrosodiethylamine (DEN) was investigated in male rats. Male Wistar rats were administered three intraperitoneal injections of DEN (200Â mg/kg bodyweight) interspersed by 2Â weeks with or without an oral dose of dimethoxy flavone (DMF 100Â mg/kg bodyweight), 2Â weeks after DEN initiation. The number of GST-Pi positive foci and proliferating cell nuclear antigen (PCNA)-positive cells were significantly suppressed by the administration of DMF. Real-time RT-PCR analysis revealed that DMF treatment increased mRNA expression levels of apoptotic proteins p53 and fas, cell cycle regulatory proteins chek 2, cdkn1a, rad 50, anti-inflammatory protein pparg whereas the mRNA expression levels of bcl-2 and prdx-2 were decreased compared to mRNA levels in DEN-treated group. Therefore, we propose that DMF partially suppresses the formation of preneoplastic lesions in rats following DEN exposure by regulating anti-inflammatory and apoptosis-promoting events and restoring the cellular redox balance altered by DEN.
Keywords
GST-pibcl 2cyclin dependent kinase inhibitor 1AN-diethylnitrosaminePPARGCDKN1ADenPCNART-PCRGAPDHFASp53DMFProliferating Cell Nuclear AntigenApoptosisdiethylnitrosaminePreneoplastic lesionsReverse transcriptase-polymerase chain reactiontumor protein 53peroxiredoxin-2glyceraldehyde-3-phosphate dehydrogenase
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Authors
Mahaboob S. Khan, Devaraj Halagowder, S Niranjali Devaraj,