Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6288583 | Food Microbiology | 2015 | 11 Pages |
Abstract
Acetic acid bacteria (AAB) usually develop biofilm on the air-liquid interface of the vinegar elaborated by traditional method. This is the first study in which the AAB microbiota present in a biofilm of vinegar obtained by traditional method was detected by pyrosequencing. Direct genomic DNA extraction from biofilm was set up to obtain suitable quality of DNA to apply in culture-independent molecular techniques. The set of primers and TaqMan - MGB probe designed in this study to enumerate the total AAB population by Real Time - PCR detected between 8Â ÃÂ 105 and 1.2Â ÃÂ 106Â cells/g in the biofilm. Pyrosequencing approach reached up to 10 AAB genera identification. The combination of culture-dependent and culture-independent molecular techniques provided a broader view of AAB microbiota from the strawberry biofilm, which was dominated by Ameyamaea, Gluconacetobacter, and Komagataeibacter genera. Culture-dependent techniques allowed isolating only one genotype, which was assigned into the Ameyamaea genus and which required more analysis for a correct species identification. Furthermore, biofilm visualization by laser confocal microscope and scanning electronic microscope showed different dispositions and cell morphologies in the strawberry vinegar biofilm compared with a grape vinegar biofilm.
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Authors
Maria José Valera, Maria Jesús Torija, Albert Mas, Estibaliz Mateo,