Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6288739 | Food Microbiology | 2014 | 7 Pages |
Abstract
This study aimed to develop a selective culture media to enumerate bifidobacteria in fermented milk and to assess this medium when used with Petrifilm⢠AC plates. For this purpose, Bifidobacterium spp., Lactobacillus spp. and Streptococcus thermophilus strains were tested to verify their fermentation patterns for different carbohydrates. All bifidobacteria strains were able to use raffinose. Based on these characteristic, a selective culture medium was proposed (Raffinose-Propionate Lithium Mupirocin, RP-MUP), used with Petrifilm⢠AC plates, and was used to enumerate bifidobacteria in fermented milk. RP-MUP performance was assessed by comparing the results with this medium to reference protocols and culture media for bifidobacteria enumeration. RP-MUP, whether used or not with Petrifilm⢠AC, presented similar performance to TOS-MUP (ISO 29981), with no significant differences between the mean bifidobacteria counts (p < 0.05) and with high correlation indices (r = 0.99, p < 0.05). As an advantage, reliable results were obtained after just 48 h of incubation when RP-MUP was used with Petrifilm⢠AC, instead of the 72 h described in the ISO 29981 protocol.
Keywords
Related Topics
Life Sciences
Agricultural and Biological Sciences
Food Science
Authors
Rodrigo Otávio Miranda, Antonio Fernandes de Carvalho, LuÃs Augusto Nero,