| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6288933 | Food Microbiology | 2013 | 9 Pages |
Abstract
⺠Seven pairs of PCR primers were designed to investigate putrescine metabolism. ⺠New touchdown PCR method was developed and tested with 32 Gram-negative bacteria. ⺠Agmatine and putrescine productions were confirmed by HPLC in all strains tested. ⺠Biogenic amine contents corresponded to the incidence of genes investigated. ⺠Sequences analyses confirmed the accuracy of new primers and the new method.
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Authors
Leona Wunderlichová, Leona BuÅková, Marek Koutný, TomáÅ¡ Valenta, FrantiÅ¡ek BuÅka,