| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6289083 | Food Microbiology | 2012 | 7 Pages |
Abstract
⺠New specific primers designed for Brochothrix thermosphacta, a seafood spoilage bacteria. ⺠Real-time PCR method optimised to quantify this bacteria in contaminated shrimp samples. ⺠Propidium monoazide treatment is added to avoid DNA amplification from dead cells. ⺠Real-time PCR quantification is correlated to microbial counts on selective medium. ⺠This method is applicable on cooked shrimp and fresh salmon.
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Authors
Kelthoum Mamlouk, Sabrina Macé, Morgan Guilbaud, Emmanuel Jaffrès, Mounir Ferchichi, Hervé Prévost, Marie-France Pilet, Xavier Dousset,