Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6289130 | Food Microbiology | 2011 | 5 Pages |
Abstract
⺠Propidium monoazide (PMA) inactivates for PCR DNA in cells with damaged membranes. ⺠DNA in E. coli killed by mild heat treatments is not inactivated by PMA. ⺠Incubation with deoxycholate renders DNA in those cells susceptible to PMA. ⺠Live E. coli at â¥1% among live and dead cells can be enumerated by real-time PCR.
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Authors
Xianqin Yang, Madhu Badoni, Colin O. Gill,