| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6289771 | International Journal of Food Microbiology | 2016 | 5 Pages |
Abstract
A simple and specific method for the rapid detection and identification of Lactobacillus brevis was developed. A fAFLP (Fluorescent Amplified Fragment Length Polymorphisms) marker for L. brevis was used to design oligonucleotide primers for a species-specific PCR assay, targeting a 125Â bp fragment of the gene encoding the aldo/keto reductase of the diketogulonate-reductase family of L. brevis. This assay resulted in 100% inclusivity and exclusivity of assignment of strains to the species L. brevis. The analytical specificity of this assay was successfully tested to identify L. brevis isolates from sourdoughs.
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Authors
Vincenzina Fusco, Grazia Marina Quero, Daniele Chieffi, Charles M.A.P. Franz,