Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6289824 | International Journal of Food Microbiology | 2015 | 22 Pages |
Abstract
Among 15 reference genes tested ALG9, FBA1, UBC6 and PFK1 appeared to be the most reliable while ENO1, PMA1, DED1 and FAS2 were the worst. The most popular reference gene ACT1, widely used for S. cerevisiae gene expression studies, showed a stability level markedly lower than those of our selected reference genes. Finally, as the expression of the new reference gene set remained constant over the entire fermentation process, irrespective of the perturbation due to sulfite addition, our results can be considered also when no sulfite is added to the must.
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Authors
Chiara Nadai, Stefano Campanaro, Alessio Giacomini, Viviana Corich,