Intelligent release of cinnamon oil from engineered proteoliposome via stimulation of Bacillus cereus protease

Inspired by the hydrolysis of casein by protease, a new approach to deliver antimicrobials against bacterial infections was developed in this study. As a natural antibacterial agent, cinnamon oil was encapsulated into engineered liposomes inlaid with casein. The average particle size of proteoliposomes was 615.0 nm and their entrapment efficiency (EE) was 40.0%. In this work, Bacillus cereus (B. cereus) was chosen as model bacterium. The controlled release of liposome-encapsulated cinnamon oil was realized via bacterial protease secreted from B. cereus. As a result, 99.99% of the bacteria could be efficiently inhibited in rice and wheat flour.