Magnetic nanoparticles replacing microplate as immobile phase could greatly improve the sensitivity of chemiluminescence enzymatic immunoassay for deoxynivalenol

•A novel chemiluminescent enzyme immunoassay was developed by adopting magnetic nanoparticle as immobile phase.•The limit of detection is low to 0.032 ng/mL.•It was pretty sensitive and the performance of this method did not decrease.

Deoxynivalenol, a secondary metabolite of Fusarium graminearum, is a suspected carcinogen. It has great threat to people's health. A sensitive determination method for deoxynivalenol is necessary to satisfy food safety requirement. In this study, traditional chemiluminescent enzyme immunoassay (CLEIA) was modified by replacing the microplate with magnetic nanoparticle as immobile phase. The limit of detection of this novel CLEIA was low to 0.032 ng/mL. The linear range was 0.1-20 ng/mL. The within-run and between-run precisions were 2.1%-4.7% and 5.4%-10.0%, respectively. And this method showed a good specificity. Therefore, it could be concluded that magnetic nanoparticles replacing microplate as immobile phase could improve the sensitivity of CLEIA with a good precision through the merits of microparticle and magnetic separation. This was significant work for food safety.