ReviewDevelopment of a novel sensitive molecularly imprinted polymer sensor based on electropolymerization of a microporous-metal-organic framework for tetracycline detection in honey

•We developed a biosensor based on molecularly imprinted polymer (MIP) microporous metal-organic framework (MMOF).•The MIP was prepared by electro-polymerization of p-aminothiophenol functionalized gold nanoparticles (Au-NPs).•The biosensor was used for the determination of Tetracycline (TC) in honey.•The limit of detection (LOD) was 2.24 × 10−16 M.•The recoveries of TC are in the range of 101.8 %−106.0 % with a relative standard deviation (RSD) ≤ 8.3%.

Electrochemical sensor for the detection of tetracycline, based on a gold electrode surface modified with a molecularly imprinted polymer microporous-metal-organic framework, is described. Imprinted gold nanoparticles composites are assembled on Au surfaces by the electropolymerization of p-aminothiophenol functionalized gold nanoparticles in the presence of the imprint molecule. The electrochemical sensor was characterized by linear sweep voltammetry in the presence of hexacyanoferrate/hexacyanoferrite as redox probe. In order to achieve optimal preconcentration, the influence of various parameters such as extraction and incubation times, washing and eluting solutions was evaluated. The sensor showed a linear range from 224 fM to 22.4 nM and a limit of detection of 0.22 fM. The proposed method was successfully applied to determine tetracycline in honey. The recoveries of tetracycline are in the range of 101.8 %−106.0 % with an RSD ≤8.3%.