Amperometric immunosensor based on deposited gold nanocrystals/4,4′-thiobisbenzenethiol for determination of carbofuran

► The deposited gold nanocrystals (DpAu)/4,4′-thiobisbenzenethiol (DMDPSE) multilayers ({DpAu/DMDPSE}n) membranes were used for modifying Au electrode to fabricate amperometric immunosensor. ► DpAu/DMDPSE multilayers were modified alternatively to form multiple membranes through Au-S bond by layer-by-layer self-assembly technology. ► The relative change in peak current (%ΔI) of the immunosensor before and after inter-action between anti-carbofuran antibody and carbofuran was mearsured by DPV technique.