| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6393534 | Food Control | 2012 | 7 Pages |
Abstract
⺠Regioisomeric haptens were used to generate novel monoclonal antibodies to picoxystrobin. ⺠Direct and indirect competitive immunoassays using homologous and heterologous antigens were optimized. ⺠Both assays were specific and showed a limit of detection around 0.1 μg/L. ⺠The new ELISAs were successfully applied to the determination of picoxystrobin in cereal flours. ⺠The described immunoreagents are promising tools for different immunoanalytical platforms.
Keywords
DMFcELISAmAbhaptenMRLHRPOVABSAN,N-dimethylformamidebovine serum albuminMonoclonal antibodyStrobilurinsOvalbuminFood safetySpacer armPesticide residuescompetitive enzyme-linked immunosorbent assayELISALOD یا Limit of detectionMaximum residue limitlimit of detectionCross-reactivityHorseradish peroxidase
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Authors
Josep V. Mercader, Javier Parra, Francesc A. Esteve-Turrillas, Consuelo Agulló, Antonio Abad-Somovilla, Antonio Abad-Fuentes,