Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6393542 | Food Control | 2012 | 6 Pages |
During 2011, 62 samples of baby formulae were checked for aflatoxin M1 (AFM1) and ochratoxin A (OTA) by immunoaffinity column (IAC) clean-up and high performance liquid chromatography with fluorescence detection (HPLC-FD). The limits of detection (LOD) and quantification (LOQ) of the analytical method were 0.005 μg kgâ1 and 0.018 μg kgâ1 for AFM1 and 0.006 μg kgâ1 and 0.021 μg kgâ1 for OTA, respectively. The recovery of AFM1 from formulae ranged between 84.27% and 88.21% (RSD = 3.54-6.18%), while the mean recoveries of OTA varied from 79.21% to 86.38% (RSD = 4.47-7.11%).Aflatoxin M1 was detected in five (8%) of formula samples, but at levels below the European legislation limit of 0.025 μg kgâ1, with concentrations ranging from 0.016 to 0.022 μg kgâ1 (mean level: 0.018 μg kgâ1). Ochratoxin A was detected in 12 out of the 62 samples (19.4%), at levels ranging from 0.017 to 0.184 μg kgâ1 (mean level: 0.103 μg kgâ1). None of the samples exceeded the maximum limit for OTA set by European Commission Regulation (0.5 μg kgâ1). The co-occurrence of AFM1 and OTA was present in only one sample (1.6%). The estimated daily intakes of AFM1 and OTA through formulae were in the range of 0.08-0.021 ng kgâ1 b.w. and 0.034-0.305 ng kgâ1 b.w., respectively. On the basis of current knowledge, it can be concluded that there is no considerable risk of AFM1 and OTA exposure for the Turkish babies and young children.
⺠AFM1 and OTA are toxic fungal metabolites of great concern in baby formulae. ⺠AFM1 and OTA levels in baby formula samples marketed in Turkey were surveyed. ⺠Samples were analyzed by IAC clean-up followed by HPLC-FD detection. ⺠No risk of AFM1 and OTA exposure for Turkish babies.