Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6394697 | Food Control | 2011 | 4 Pages |
Abstract
In order to detect nodularin (NOD) from water and fish samples, a monoclonal antibody (mAb) specific for NOD was selected which has no cross-reactivity with other toxins (<0.4%). Based on the mAb, a competitive enzyme-linked immunosorbent assay (ELISA) was developed. The working range of the ELISA was from 0.5 to 100 ng mLâ1 with a detection limits of 0.16 ng mLâ1. The average recoveries of NOD from sea water, flounder and blue mussels were 87.8%, 90.7% and 86.9%, respectively with the coefficients of variation (CVs) from 2.1% to 3.2%. Therefore, the developed competitive ELISA may become a new convenient and satisfied analytical tool for detecting of NOD in water and fish samples.
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Authors
Yu Zhou, Yan-Song Li, Bai-Hui Zhi, Shi-Ying Lu, Hong-Lin Ren, Yuan-Yuan Zhang, Zhao-Hui Li, Qing-Feng Shen, Xian-Mei Meng, Zeng-Shan Liu, Jun-Hui Zhang, Ya-Ming Hao, Wen-Di Liu, Fang Song, Dong-Ming Yan,