Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6397519 | Food Research International | 2007 | 7 Pages |
â¢Enzymatic phenol oxidation may occur in virgin olive oil in contact with vegetables.â¢Laccase oxidation of polyphenols within the virgin olive oil (VOO) was studied.â¢Laccase led to a significant reduction of oleuropein and o-diphenols fraction.â¢Controversial results were found during the accelerated oil oxidation trials.â¢Enzymatic phenol oxidation into VOO may be exploited also for analytical purposes.
Activity of laccase from Trametes versicolor was assayed directly in virgin olive oil (VOO) samples. Laccase-treated oils led to the formation of insoluble precipitate and to significant qualitative and quantitative changes of their polyphenol composition. At the extreme condition of oil/laccase incubation (60 °C for 1 h) depletion of oleuropein and o-diphenols was estimated up to 90% and 77%, respectively. Results of Rancimat test (130 °C and 20 L hâ 1) and oven-test (60 °C) evidenced controversial effects on the VOO oxidative stability. At high temperature, induction time of laccase-treated oils was found to be similar or even lower than the one recorded in the chemically-dephenolized oils; therefore, enzyme treatment induced a pro-oxidant effect. Conversely, during the storage at 60 °C, all the laccase-treated oils showed an improvement on their oxidative stability compared to the fresh oil counterparts. Experimental data suggest that polyphenol enzymatic-oxidation generated oil by-products which manifest higher radical scavenging or conversely pro-oxidant property, depending on the treatment temperature and storage conditions of the oils.