| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6398604 | Food Research International | 2012 | 8 Pages |
This paper reports a physiologically relevant and rapid mitochondria-based assay for assessing antioxidant properties of pure compounds and herb extracts. The principle of this method is antioxidants can scavenge peroxyl radicals generated in mitochondria thus to inhibit 2â²,7â²-dichlorofluorescin diacetate oxidation. Ascorbic acid, chlorogenic acid and berberine showed a dose-dependent antioxidative capacity; interestingly, lutein, Rehmannia glutinosa and Scrophularia ningpoensi exhibited pro-antioxidant properties. The antioxidant properties of herb extracts obtained by this method are in good agreement with the results measured by cell-based assay (r = 0.931, p < 0.001). This novel assay has acceptable accuracy, precision, reproducibility and the mitochondria-based assay may be more useful than conventional assay for detecting antioxidant capacity in a variety of foods.
⺠A new mitochondria-based assay was proposed. ⺠This method can be used to assess the pro- and antioxidant properties of samples. ⺠The assay is more physiologically relevant than conventional chemical method. ⺠The assay has acceptable accuracy, precision and reproducibility.
