Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6400782 | LWT - Food Science and Technology | 2015 | 8 Pages |
â¢Two major canola proteins, cruciferin and napin, have unique functional and biological properties.â¢An integrated method of isolating cruciferin and napin was developed.â¢The purity of cruciferin and napin were 91 and 82 g/100 g, respectively.â¢The yield of cruciferin and napin were 38.6% and 12.5%, respectively.
Canola proteins are known to have great potential for use in food and non-food applications due to their nutritional, biological and functional properties. However, canola protein extraction remains a challenge, due mainly to the complexity of canola proteins and the presence a variety of undesirable compounds. An integrated method, including acidic washing (pH 4), alkaline extraction (pH 12.5), isoelectric precipitation (pH 4), and ultrafiltration, was proposed to isolate two main canola proteins: cruciferin and napin. The protein content and yield of cruciferin and napin were 91Â g/100Â g and 38.6%, 82Â g/100Â g and 12.5%, respectively. The contents of phytic acid and phenolics were 1.5Â g/100Â g and 1.8Â g/100Â g, and 0.9Â g/100Â g and 0.6Â g/100Â g, respectively, for cruciferin and napin. The emulsifying and foaming properties of cruciferin and napin were favorably comparable to previous results. The development of a simple and scalable method of canola protein isolation will facilitate further research on their food and non-food applications.