Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6401230 | LWT - Food Science and Technology | 2015 | 7 Pages |
Abstract
Three antioxidant fractions (Fr1, Fr2 and Fr3) were separated by solid phase extraction from dried bilberry (Vaccinium myrtillus L.) and tested for antioxidant and antiproliferative activity. Vitamin C (1.02Â mg/100Â g) was identified in Fr1, six flavonoids in Fr2 and eight phenolic acids in Fr3. The most abundant compounds in Fr2 and Fr3 were quercetin (243.3Â mg/100Â g dried bilberry) and p-coumaric acid (57.87 mg/100Â g dried bilberry), respectively. The best free radical scavenging activity on hydroxyl radicals was expressed by Fr3 (EC50â
OHÂ =Â 0.117Â mg/ml), and on 2,2-diphenyl-1-picrihydrazyl (DPPH) radicals by Fr2 (EC50DPPHâ
 = 0.025 mg/ml). Considering the kinetic behaviour, tested by spectrophotometrical DPPH method, the highest antiradical efficiency (AE = 19.82 ml/mg Ã min) was demonstrated by Fr2. Fr2 and Fr3 significantly inhibited the growth of cervix epitheloid carcinoma, breast adenocarcinoma and colon adenocarcinoma cell lines, with IC50 ranging from 125.80 to 300.48 μg/ml. Dried bilberry possesses beneficial effects in prevention of cancer and diseases which have been attributed to free radical production.
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Authors
Vesna Tumbas Å aponjac, Jasna ÄanadanoviÄ-Brunet, Gordana ÄetkoviÄ, Sonja Djilas, Dragana ÄetojeviÄ-Simin,