Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6404811 | LWT - Food Science and Technology | 2013 | 5 Pages |
The optimized extraction conditions of red pigments (RP) from bayberry (Myrica rubra) pomace obtained with response surface methodology were extraction pH, time, temperature, liquid/solid ratio as 1.4, 4.2 h, 56.1 °C and 12.6/1 ml/g, respectively. The RP were then purified by the macroporous resin, which indicated the resin LX-68 showed the maximum refined efficiency among the tested resins. Further solvent partition of the RP yielded ethyl acetate, n-butanol and water layers, in which the first fraction showed the strongest DPPH and hydroxyl radical scavenging activities with IC50 values of 2.13 μg/ml and 0.57 mg/ml, respectively, which were higher than those of BHA and mannitol used as a positive control. Following activity-oriented separation, (â)-epicatechin and procyanidin B2 were isolated from the ethyl acetate layer and were found to have excellent DPPH free radical scavenging activities with IC50 values of 2.87 and 2.06 μg/ml, respectively.
⺠We got the optimized extraction conditions of red pigments from bayberry pomace. ⺠The resin LX-68 was suitable for purifying the red pigments from bayberry pomace. ⺠The ethyl acetate layer showed the strongest bioactivities in tested three fractions. ⺠(â)-epicatechin and procyanidin B2 were isolated as major antioxidant metabolites.