Electro-membrane fractionation of antioxidant peptides from protein hydrolysates of rainbow trout (Oncorhynchus mykiss) byproducts

The electro-membrane fractionation developed in this study is a two-step process, which is able to selectively separate antioxidant peptides from enzymatic protein hydrolysates based on charge and size. With this particular raw material as the feed, we have shown that using this approach lead to the highest peptide migration rate and a significant improvement in antioxidant activities of both peptide fractions. In addition, this technique is very selective, and environmental friendly as it requires no use of solvent and consumes less energy compared to conventional chromatographic techniques, and thus can be used as a green technology for the fractionation of bioactive peptides from a complex mixture of protein hydrolysates.