Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8546567 | Food and Chemical Toxicology | 2018 | 35 Pages |
Abstract
Patulin (PAT), deoxynivalenol (DON) and toxin T-2 (T-2) are mycotoxins distributed worldwide in food and feed. Cytotoxicity of the three mycotoxins individually or in combination in human hepatocellular carcinoma (HepG2) cells was evaluated by MTT assay over 24, 48 and 72â¯h of exposure. The concentration ranges used were 0.625-15â¯Î¼M for DON, 1.25-50â¯nM for T-2 and 0.45-7.5â¯Î¼M for PAT. The IC50 values obtained ranged from 9.30 to 2.53 μM, from 33.69 to 44.37 nM and from 2.66 to 1.17 μM for DON, T-2 and PAT, respectively. The most cytotoxic mycotoxin to HepG2 cells was T-2 followed by PAT and DON. The combination ratios used for the mixtures were 1:3 (DON: T-2), 1:5 (DON: PAT), 1:1.7 (T-2: PAT) and 1:3:5 (DON: T-2: PAT). The mixture with the highest cytotoxic effect was T-2+PAT, followed by DON + T-2+PAT, DON + T-2 and DON + PAT respect to the cytotoxic effect of their individuals. In the combinations, at low fa an antagonistic effect was detected, and this effect changes the shape of the combination to additive effect at high fa in the mixtures.
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Authors
Celia Fernández-Blanco, Luigi Elmo, Thomas Waldner, Maria-Jose Ruiz,