Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8546812 | Food and Chemical Toxicology | 2018 | 28 Pages |
Abstract
Acrylamide (AA) occurs in many cooked carbohydrate-rich foods and has caused widespread concern as a possible carcinogen. Glycidamide (GA) is the ultimate genotoxic metabolite of AA. The present study was to investigate the protective effect of Cyanidin-3-O-glucoside (C3G) against AA- and GA-induced reproductive toxicity in R2C Leydig cells. The results demonstrated that C3G inhibited AA- and GA-induced cytotoxicity and mitochondria-mediated cell apoptosis, the effective doses of C3G were ranging from 10 to 50â¯Î¼M. Besides, AA (1.925â¯mM) and GA (0.872â¯mM) exposure increased ROS level and decreased mitochondrial membrane potential, which led to a decrease in progesterone production, while C3G ranging from 10 to 50â¯Î¼M reduced ROS immediately, and increased progesterone production after 24â¯h treatment. Furthermore, C3G up-regulated expression of Bcl-2 protein and down-regulated pro-apoptotic protein Bax and cleaved Caspase-3 after 24â¯h treatment in 1.925â¯mM AA- and 0.872â¯mM GA-treated R2C cells. Moreover, C3G intervention increased the protein expression of steroidogenic acute regulatory (StAR). It was concluded that C3G is effective in reducing AA- and GA-induced reproductive toxicity via inhibition of ROS generation, mitochondrial membrane depolarization and apoptosis, as well as activating steroidogenic enzymes.
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Authors
Jianxia Sun, Mingwei Li, Feiyan Zou, Shun Bai, Xinwei Jiang, Lingmin Tian, Shiyi Ou, Rui Jiao, Weibin Bai,