Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8547116 | Food and Chemical Toxicology | 2018 | 28 Pages |
Abstract
The cell growth inhibitory activity of the hydroethanolic extract of Achillea millefolium was studied in human tumor cell lines (NCI-H460 and HCT-15) and its mechanism of action was investigated. The GI50 concentration was determined with the sulforhodamine B assay and cell cycle and apoptosis were analyzed by flow cytometry following incubation with PI or Annexin V FITC/PI, respectively. The expression levels of proteins involved in cell cycle and apoptosis were analyzed by Western blot. The extracts were characterized regarding their phenolic composition by LC-DAD-ESI/MS. 3,5-O-Dicaffeoylquinic acid, followed by 5-O-caffeoylquinic acid, were the main phenolic acids, while, luteolin-O-acetylhexoside and apigenin-O-acetylhexoside were the main flavonoids. This extract decreased the growth of the tested cell lines, being more potent in HCT-15 and then in NCI-H460â¯cells. Two different concentrations of the extract (75 and 100 μg/mL) caused alterations in cell cycle profile and increased apoptosis levels in HCT-15 and NCI-H460â¯cells. Moreover, the extract caused an increase in p53 and p21 expression in NCI-H460â¯cells (which have wt p53), and reduced XIAP levels in HCT-15â¯cells (with mutant p53). This work enhances the importance of A. millefolium as source of bioactive phenolic compounds, particularly of XIAP inhibitors.
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Authors
Joana M. Pereira, Vanessa Peixoto, Alexandra Teixeira, Diana Sousa, Lillian Barros, Isabel C.F.R. Ferreira, M. Helena Vasconcelos,