Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8548303 | Food and Chemical Toxicology | 2018 | 22 Pages |
Abstract
Sitagliptin is an active ingredient of antidiabetic drug used in the treatment of type 2 diabetes mellitus (T2DM). In this study, the genotoxic effects of sitagliptin were determined in human lymphocytes by using chromosome aberrations (CAs), sister chromatid exchanges (SCEs), micronucleus (MN) and comet assays. 31.25-1000â¯Î¼g/mL concentrations of sitagliptin were used. Sitagliptin significantly increased the frequency of CAs and SCEs at the highest concentration at 24â¯h treatment and all concentrations (except 250â¯Î¼g/mL for CA, except 31.25 and 62.50â¯Î¼g/mL for SCE) at 48â¯h treatment compared with solvent control (DMSO). This compound increased the MN at only the highest concentration compared with the solvent control. Mitotic index (MI) significantly decreased at the three highest concentrations of sitagliptin at 48â¯h treatment. However, replication (RI) and nuclear division (NDI) indices were not affected at all the treatments. Comet assay results indicated that sitagliptin significantly increased mean comet tail intensity and tail moment at only two concentrations (62.50 and 1000â¯Î¼g/mL for intensity, 125 and 1000â¯Î¼g/mL for tail moment), and tail length at all concentrations (except 125 and 500â¯Î¼g/mL). It was concluded that higher concentration of sitagliptin had genotoxic effects in the human lymphocytes in vitro.
Keywords
MMCNDICASSCGESCEsGLP-1GIPT2DMmitomycin-CDPP-4Chromosome aberrationsSingle Cell Gel Electrophoresissister chromatid exchangesAntidiabetic drugbinucleateType 2 diabetes mellitusDipeptidyl peptidase-4GenotoxicitySitagliptinnuclear division indexreplication indexmitotic indexHuman lymphocytesMicronucleusglucose-dependent insulinotropic polypeptide
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Food Science
Authors
Deniz Yuzbasioglu, Cemile Enguzel-Alperen, Fatma Unal,