Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8887659 | Food Control | 2019 | 10 Pages |
Abstract
Spinosad has been extensively used for insect control in agricultural crops. In the present study, a novel succinic derivative of spinosyn A as the hapten was synthesized, and an indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed using a new monoclonal antibody (designed as mAb3E6) specifically against spinosyn A. A mAb3E6-based immunoaffinity column was developed for enrichment extraction of spinosyn A and reduction of matrix interference. The half maximum inhibitory concentration and limit of detection (LOD) of the icELISA were 4.11â¯ng/mL and 0.63â¯ng/mL, respectively. The icELISA had average fortified recoveries from 78.1% to 103.2% and the validation results correlated well with high-performance liquid chromatography. The mAb3E6 showed cross reactivity of 80.4%, 75.7% and less than 0.1% with spinosyns B, D and other macrolides, respectively. Interactions of the antibody and macrolides were further analyzed by molecular docking. The developed immunoaffinity chromatography-assisted icELISA is potentially a sensitive and convenient tool for monitoring spinosad residues in milk, fruits, and vegetables.
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Authors
Jianqiang Lan, Hongwei Zhao, Xiaotuo Jin, Huai Guan, Yuan Song, Yongmei Fan, Xiaoping Diao, Baomin Wang, Qian Han,