Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8890703 | LWT - Food Science and Technology | 2018 | 7 Pages |
Abstract
Essential oils (EO) have been studied as a possible substitute of fungicides. EO have been loaded in polymeric nanoparticles to prevent their degradation and enhance antifungal activity. In this work was to prepared chitosan nanoparticles with Schinus molle EO by ionotropic gelation. The bionanocomposites were characterized by scanning electronic microscopy (SEM), dynamic light scattering (DLS) and Fourier transform infrared spectroscopy (FTIR). The antifungal activity was evaluated in different growth stages of A. parasiticus (spore germination, radial growth and spore viability) and the effect in aflatoxin production was analyzed by using affinity chromatography columns from VICAM. DLS results showed that the Np-CS and Np-CS-SEO diameters were 361.9â¯Â±â¯31.4 and 516.9â¯Â±â¯45.4â¯nm, respectively. According to SEM, the NPs presented spherical shape and the zeta potential was slightly decreased when Schinus molle EO was incorporated. The Np-CS-SEO (62.5-500â¯Î¼gâ¯mLâ1) showed a high inhibition (>80%) on spore germination of A. parasiticus at 12â¯h of incubation, while, radial growth was not inhibited for these NPs. At a concentration of 500â¯Î¼gâ¯mLâ1, Np-CS-SEO inhibited up to 59% the aflatoxin production. Depending on concentration, the Np-CS-SEO can be considered as an alternative to control A. parasiticus.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Food Science
Authors
A.K. López-Meneses, M. Plascencia-Jatomea, J. Lizardi-Mendoza, D. Fernández-Quiroz, F. RodrÃguez-Félix, R.R. Mouriño-Pérez, M.O. Cortez-Rocha,