Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8890903 | LWT - Food Science and Technology | 2018 | 42 Pages |
Abstract
A heteropolysaccharide BCP-2 was isolated from blackcurrant fruits by successively using D4006 resin, aqueous two phase system, and Sephadex G-100 column chromatography. Its inhibitory activities against α-glucosidase, α-amylase and glycation were investigated. BCP-2 was found to be composed of galacturonic acid, rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 1.3:2.5:5.1:1.8:1.0:72.0:4.5 with molecular weight of 2.18â¯Ãâ¯104â¯kDa. The glycosyl linkages of (1â¯ââ¯3)-α-l-Rhamnose, (1â¯ââ¯4)-β-d-Mannose, (1â¯ââ¯6)-α-d-Galactose and (1â¯ââ¯4)-α-d-Glucose were confirmed in BCP-2. BCP-2 had no triple-helix conformation and possessed a honeycomb structure with an irregular branching shape. BCP-2 showed moderate α-amylase inhibitory activity (37.93%) and clear α-glucosidase inhibitory activity (78.42%). The inhibitory effect of BCP-2 on α-amylase was reversible and exhibited a competitively inhibitory process. Besides, BCP-2 exerted more significant anti-glycation activities than aminoguanidine during 15 days of incubation. Specifically, the inhibitory abilities were more effective on the formation of dicarbonyl compounds (66.54%) and AGEs (70.24%) than that of Amadori products (37.03%). These results suggested that BCP-2 may be a potential anti-diabetic agent.
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Authors
Yaqin Xu, Yingying Guo, Yankang Gao, Xiaojie Niu, Libo Wang, Xingguo Li, Hongchao Chen, Zeyuan Yu, Yu Yang,