Growth factors affecting gas production and reduction potential of vegetative cell and spore inocula of dairy-related Clostridium species

Gas production resulting from C. tyrobutyricum spore germination and growth was not affected by acidity and salt concentration at 20 °C. Some strains of C. sporogenes were capable of producing gas even at 12 °C, pH 5.00 and 1% of NaCl. A ripening temperature below 10 °C represents a potential strategy to prevent LBD occurrence.