Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8891245 | LWT - Food Science and Technology | 2018 | 7 Pages |
Abstract
High purity diacylglycerols (DAG) rich in medium-chain fatty acid diacylglycerols (MCD) and medium- and long-chain fatty acid diacylglycerols (MLCD) were prepared via the enzymatic esterification of monoacylglycerols (MAG) with caprylic acid followed by molecular distillation (MD), solvent fraction and low-temperature centrifugation. The content of DAG in the crude product was 44.8â¯Â±â¯0.1%, under the selected esterification conditions, which were MAGs/caprylic acid mole ratio of 1:3, reaction temperature of 65â¯Â°C, reaction time of 30â¯min and enzyme load of 5â¯wt%. Subsequently, the one-step MD and solvent fraction in methanol/ethanol increased the DAG content to 61.3â¯Â±â¯0.8%. Eventually, the product containing 86.6â¯Â±â¯0.6% of DAG with 39.3â¯Â±â¯1.3% of MCD and 47.3â¯Â±â¯0.6% of MLCD was obtained by the methanol crystallization at 0â¯Â°C with a water content of 9â¯wt% and a 1:3 ratio of glycerides/methanol (v/v) followed by the centrifugation separation at 0â¯Â°C.
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Authors
Guanghui Li, Jiazi Chen, Xiang Ma, Zhen Zhang, Ning Liu, Yong Wang,