Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8891444 | LWT - Food Science and Technology | 2018 | 30 Pages |
Abstract
Probiotic application in food industry remains a challenge in decades because of bacterial loss in viability during powder manufacture and storage. Well-designed cryoprotectants and storage condition are crucial for successful probiotic application. In this study, survival of Lactobacillus acidophilus with composite cryoprotectants was investigated during freeze-drying and subsequent storage at different temperature (â18 °C, 4 °C and room temperature- 25 °C). The composite cryoprotectants were optimized to be trehalose of 13%, Na2HPO4 of 0.33%, lactose of 7.5% and skim milk powder of 21% with the aid of response surface method. The survival rate of freeze-dried L. acidophilus increased to (93.9 ± 0.12) % in comparison to control (36.6 ± 0.08) %. Furthermore, freeze-dried L. acidophilus powder was applied in two dairy forms (goat milk powder and tablets) and cell viability was monitored during 180 days of storage. The freeze-dried powder exhibited better stability in goat milk powder than that in tablets. The cell survival in goat milk powder and tablets was higher at low temperature (â18 °C and 4 °C) during storage. Our finding provided a formulation of composite cryoprotectant with great protective effect to probiotic cells during freeze-drying. The freeze-dried powder showed potential application to functional dairy production in relative food industry.
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Authors
Guowei Shu, Zhuo Wang, Li Chen, Hongchang Wan, He Chen,