Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8979170 | International Dairy Journal | 2005 | 8 Pages |
Abstract
Polymerase chain reaction (PCR) has been applied for the specific detection of cows' DNA in water buffalo milk and mozzarella cheese by using primers targeting the mitochondrial 12S ribosomal RNA gene. The use of specific primers for cow yielded a 346Â bp fragment from cows' milk DNA, whereas no amplification signal was obtained in sheep's, goats' and water buffalo's milk DNA. Analysis of both buffalo milk and buffalo mozzarella cheese mixtures containing different percentages of cows' milk or bovine mozzarella cheese, enabled the specific detection of cow's DNA with a sensitivity threshold of 0.1%. The proposed PCR assay represents a rapid and straightforward method for the detection of adulterations in water buffalo milk and mozzarella cheese.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Food Science
Authors
I. López-Calleja, I. González Alonso, V. Fajardo, M.A. RodrÃguez, P.E. Hernández, T. GarcÃa, R. MartÃn,