Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9030856 | Food and Chemical Toxicology | 2005 | 9 Pages |
Abstract
Previously, we reported that a 1-butanol soluble extract of the edible red alga Palmaria palmata, known as dulse, exhibited hydroxyl and stable free radical scavenging activity as well as inhibition of lipid peroxidation, attributed to the reducing activity and polyphenol content of the dulse extract. In the present study, we evaluated the antioxidant and antiproliferative activities of two grades of dulse harvested from Canadian Maritime locations differing in UV radiation exposure (i.e. west versus east coasts of Grand Manan Island, New Brunswick). The 1-butanol soluble extract from Grade 1 dulse (reduced UV-exposure) exhibited lower reducing activity versus Grade 2 dulse (greater UV exposure) reflecting a lower requirement for endogenous antioxidant protection. Grade 1 and 2 dulse extracts both inhibited (p ⩽ 0.03) AAPH-induced lipid peroxidation, but had no effect on AMVN-induced lipid peroxidation, demonstrating the aqueous nature of the antioxidants involved. The Grade 1 and 2 dulse extract inhibition (p < 0.05) of HeLa cell proliferation was dose-dependent over 0.5-5.0 mg/mL and maximal at 48 and 72 h incubation. The antiproliferative effects of the Grade 1 and 2 dulse extracts in the present study likely reflect the bioactivity of the polyphenol content of these extracts.
Keywords
MTT, 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromideAAPHTBARS, 2-thiobarbituric acid reactive substancesPalmaria palmataAMVNAMVN, 2,2′-azobis(2,4-dimethylvaleronitrile)BHA, butylated hydroxyanisoleEGC, epigallocatechinFBS, foetal bovine serumMDA, malondialdehydeMEM, minimum essential mediumOH, hydroxyl radicalROS, Reactive Oxygen SpeciesTCA, trichloroacetic acidLipid oxidationCell proliferationDulseHeLa cellsCD, conjugated dienesAntioxidant activityPBS, phosphate buffered salineGSH, glutathione
Related Topics
Life Sciences
Agricultural and Biological Sciences
Food Science
Authors
Yvonne V. Yuan, Meshell F. Carrington, Natalie A. Walsh,