Application of tristimulus colorimetry to optimize the extraction of anthocyanins from Jaboticaba (Myricia Jaboticaba Berg.)

This paper was aimed at optimizing the extraction conditions for the maximum yield of anthocyanins from Jaboticaba (Myricia Jaboticaba Berg.) skins. Tristimulus colorimetry was applied to evaluate quantitatively and qualitatively the process. A mixed 2 and 3 full factorial design was used (21 · 33). The factors and levels studied were type of solvent: ethanol or methanol; solvent/water mixture: 100% v/v, 90% v/v, 80% v/v; type of acid: clorhidric acid, citric acid or acetic acid and pH: 2, 3 or 4. The estimated effect and ANOVA parameters were calculated. The yield of anthocyanins, quantified following an spectrophotometric method based on the absorbance at 535 nm was 92.92 ± mg/kg of skin. The yield of anthocyanins showed a high and significant correlation with chroma (Cab∗) (p < 0.05; r = 0.84) and lightness (L*) (p < 0.05; r = −0.85) and it was significantly (p < 0.001) affected by the type of acid used and the solvent/water% (p < 0.05).