In silico aided metabolic engineering of Saccharomyces cerevisiae for improved bioethanol production Fulltext Access 10 Pages 2006
Secretory expression of synthetic human Fas ligand extracellular domain gene in Pichia pastoris: Influences of tag addition and N-glycosylation site deletion, and development of a purification method Fulltext Access 10 Pages 2006
Spatial distribution of fructans and fructan metabolizing enzymes in rhizophores of Vernonia herbacea (Vell.) Rusby (Asteraceae) in different developmental phases Fulltext Access 10 Pages 2006
A putative flavin-containing mono-oxygenase as a marker for certain defense and cell death pathways Fulltext Access 10 Pages 2006
Immediate responses and adaptative strategies of three olive cultivars under contrasting water availability regimes: Changes on structure and chemical composition of foliage and oxidative damage Fulltext Access 10 Pages 2006
Phytochelatins are only partially correlated with Cd-stress in two species of Brassica Fulltext Access 10 Pages 2006
The fgr gene responsible for rice fragrance was restricted within 69 kb Fulltext Access 10 Pages 2006
Deglycosylation to obtain stable and homogeneous Pichia pastoris-expressed N–A1 domains of carcinoembryonic antigen Fulltext Access 10 Pages 2006
Swelling and de-swelling kinetics of gelatin hydrogels in ethanol–water marginal solvent Fulltext Access 10 Pages 2006
Effects of pH and ionic strength on the assembly and bundling of FtsZ protofilaments: A possible role of electrostatic interactions in the bundling of protofilaments Fulltext Access 10 Pages 2006
Expression profiling of genes involved in paclitaxel biosynthesis for targeted metabolic engineering Fulltext Access 10 Pages 2006
Functional expression of a P450 flavonoid hydroxylase for the biosynthesis of plant-specific hydroxylated flavonols in Escherichia coli Fulltext Access 10 Pages 2006
Effects of elevated ammonium on glycosylation gene expression in CHO cells Fulltext Access 10 Pages 2006
Expression of recombinant hybrid peptide cecropinA(1–8)–magainin2(1–12) in Pichia pastoris: Purification and characterization Fulltext Access 10 Pages 2006
Replacement of the glucose phosphotransferase transport system by galactose permease reduces acetate accumulation and improves process performance of Escherichia coli for recombinant protein production without impairment of growth rate Fulltext Access 10 Pages 2006
Introducing glutathione biosynthetic capability into Lactococcus lactis subsp. cremoris NZ9000 improves the oxidative-stress resistance of the host Fulltext Access 10 Pages 2006
Performance and microbial communities of a continuous stirred tank anaerobic reactor treating two-phases olive mill solid wastes at low organic loading rates Fulltext Access 10 Pages 2006
Functional large-scale production of a novel Jonesia sp. xyloglucanase by heterologous secretion from Streptomyces lividans Fulltext Access 10 Pages 2006
High level expression of monomeric and dimeric human α1,3-fucosyltransferase V Fulltext Access 10 Pages 2006
Affinity binding of inclusion bodies on supermacroporous monolithic cryogels using labeling with specific antibodies Fulltext Access 10 Pages 2006
Stress-related differential expression of multiple β-carotene ketolase genes in the unicellular green alga Haematococcus pluvialis Fulltext Access 10 Pages 2006
Construction of random transposition mutagenesis system in Rhodococcuserythropolis using IS1415 Fulltext Access 10 Pages 2006
Net P-removal deterioration in enriched PAO sludge subjected to permanent aerobic conditions Fulltext Access 10 Pages 2006
A nanocompartment system (Synthosome) designed for biotechnological applications Fulltext Access 10 Pages 2006
Purification and characterization of a novel pectinase from Acrophialophora nainiana with emphasis on its physicochemical properties Fulltext Access 10 Pages 2006
An efficient mammalian cell-free translation system supplemented with translation factors Fulltext Access 10 Pages 2006
Combination of 3D tissue engineered scaffold and non-viral gene carrier enhance in vitro DNA expression of mesenchymal stem cells Fulltext Access 10 Pages 2006
Nanoparticles of cationic amphiphilic cyclodextrins entangling anionic porphyrins as carrier-sensitizer system in photodynamic cancer therapy Fulltext Access 10 Pages 2006
Characterization of recombinant Francisella tularensis acid phosphatase A Fulltext Access 10 Pages 2006
High levels of protein expression using different mammalian CMV promoters in several cell lines Fulltext Access 10 Pages 2006
Development of the system ensuring a high-level expression of hepatitis C virus nonstructural NS5B and NS5A proteins Fulltext Access 10 Pages 2006
Cloning, expression, and purification of a novel recombinant antigen from Leishmania donovani Fulltext Access 10 Pages 2006
An optimized system for expression and purification of secreted bacterial proteins Fulltext Access 10 Pages 2006
Efficient production of a folded and functional, highly disulfide-bonded β-helix antifreeze protein in bacteria Fulltext Access 10 Pages 2006
Expression and purification of dalcochinase, a β-glucosidase from Dalbergia cochinchinensis Pierre, in yeast and bacterial hosts Fulltext Access 10 Pages 2006
Dot-blot immunodetection as a versatile and high-throughput assay to evaluate recombinant GPCRs produced in the yeast Pichia pastoris Fulltext Access 10 Pages 2006
Production and characterization of clinical grade Escherichia coli derived Plasmodium falciparum 42 kDa merozoite surface protein 1 (MSP142) in the absence of an affinity tag Fulltext Access 10 Pages 2006
Preparation of isotopically labeled spinach acyl–acyl carrier protein for NMR structural studies Fulltext Access 10 Pages 2006
Glucose oxidase immobilization on a novel cellulose acetate–polymethylmethacrylate membrane Fulltext Access 10 Pages 2006
An efficient method to express and refold a truncated human procaspase-9: A caspase with activity toward Glu-X bonds Fulltext Access 10 Pages 2006
Production and purification of self-assembling peptides in Ralstonia eutropha Fulltext Access 10 Pages 2006
A novel recombinant multiepitope protein as a hepatitis C diagnostic intermediate of high sensitivity and specificity Fulltext Access 10 Pages 2006
Challenges in the expression of disulfide bonded, threonine-rich antifreeze proteins in bacteria and yeast Fulltext Access 10 Pages 2006
An extracellular exo-β-(1,3)-glucanase from Pichia pastoris: Purification, characterization, molecular cloning, and functional expression Fulltext Access 10 Pages 2006
Cloning, overexpression, and characterization of a thermoactive nitrilase from the hyperthermophilic archaeon Pyrococcus abyssi Fulltext Access 10 Pages 2006
A novel fusion protein system for the production of native human pepsinogen in the bacterial periplasm Fulltext Access 10 Pages 2006
High-level expression and purification of human thymidine kinase 1: Quaternary structure, stability, and kinetics Fulltext Access 10 Pages 2006
Establishment of a yeast system that stably expresses human cytochrome P450 reductase: Application for the study of drug metabolism of cytochrome P450s in vitro Fulltext Access 10 Pages 2006
Purification, characterization, and mass spectrometric sequencing of a medium chain acyl-CoA synthetase from mouse liver mitochondria and comparisons with the homologues of rat and bovine Fulltext Access 10 Pages 2006
Expression and purification of sea raven type II antifreeze protein from Drosophila melanogaster S2 cells Fulltext Access 10 Pages 2006
Synthesis and characterization of polyaminoacidic polycations for gene delivery Fulltext Access 10 Pages 2006
Electrical coupling of cardiomyocyte sheets occurs rapidly via functional gap junction formation Fulltext Access 10 Pages 2006
Biomineralization of calcium disilicide in porous polycaprolactone scaffolds Fulltext Access 10 Pages 2006
Heparin-regulated release of growth factors in vitro and angiogenic response in vivo to implanted hyaluronan hydrogels containing VEGF and bFGF Fulltext Access 10 Pages 2006
In vitro studies of annulus fibrosus disc cell attachment, differentiation and matrix production on PDLLA/45S5 Bioglass® composite films Fulltext Access 10 Pages 2006
Biomimetic phosphorylcholine polymer grafting from polydimethylsiloxane surface using photo-induced polymerization Fulltext Access 10 Pages 2006
Co-culture of human embryonic stem cells with murine embryonic fibroblasts on microwell-patterned substrates Fulltext Access 10 Pages 2006
Activation of phospholipase D1 by surface roughness of titanium in MG63 osteoblast-like cell Fulltext Access 10 Pages 2006
The generation of germline transgenic silkworms for the production of biologically active recombinant fusion proteins of fibroin and human basic fibroblast growth factor Fulltext Access 10 Pages 2006
Elastogenic effects of exogenous hyaluronan oligosaccharides on vascular smooth muscle cells Fulltext Access 10 Pages 2006
Long-term maintenance of human hepatocytes in oxygen-permeable membrane bioreactor Fulltext Access 10 Pages 2006
Effect of biomaterial surface properties on fibronectin–α5β1 integrin interaction and cellular attachment Fulltext Access 10 Pages 2006
Synthesis and evaluation of poly(diol citrate) biodegradable elastomers Fulltext Access 10 Pages 2006
The in vitro hydrolysis of poly(ester urethane)s consisting of poly[(R)-3-hydroxybutyrate] and poly(ethylene glycol) Fulltext Access 10 Pages 2006
Injectable and self-curing composites of acrylic/bioactive glass and drug systems. A histomorphometric analysis of the behaviour in rabbits Fulltext Access 10 Pages 2006
PEO–PPO–PEO-based poly(ether ester urethane)s as degradable reverse thermo-responsive multiblock copolymers Fulltext Access 10 Pages 2006
Dynamic mechanical studies of hydrolytic degradation in isotropic and oriented Maxon B Fulltext Access 10 Pages 2006
A novel glass slide-based peptide array support with high functionality resisting non-specific protein adsorption Fulltext Access 10 Pages 2006
Proliferation and differentiation of neural stem cells on lysine–alanine sequential polymer substrates Fulltext Access 10 Pages 2006
Effect of cobalt and chromium ions on human MG-63 osteoblasts in vitro: Morphology, cytotoxicity, and oxidative stress Fulltext Access 10 Pages 2006
K562 cell growth activity and metabolism characteristics in APA microencapsulated culture and modeling study Fulltext Access 10 Pages 2006
Production of cellulases by Penicillium brasilianum IBT 20888—Effect of substrate on hydrolytic performance Fulltext Access 10 Pages 2006
In situ measurement of activity and mass transfer effects in enzyme immobilized electrodes Fulltext Access 10 Pages 2006
Acyl transfer strategy for the biocatalytical characterisation of Candida rugosa lipases in organic solvents Fulltext Access 10 Pages 2006
Enantioselective microbial reduction of 6-oxo-8-[4-[4-(2-pyrimidinyl)-1-piperazinyl]butyl]-8-azaspiro[4.5]decane-7,9-dione: Cloning and expression of reductases Fulltext Access 10 Pages 2006
Cloning, expression, and partial characterization of a family B-type DNA polymerase from the hyperthermophilic crenarchaeon Sulfophobococcus zilligii Fulltext Access 10 Pages 2006
Granular sludge in full-scale anaerobic bioreactors: Trace element content and deficiencies Fulltext Access 10 Pages 2006
Tumour cell adhesion and integrin expression affected by Ganoderma lucidum Fulltext Access 10 Pages 2006
Engineering drought tolerance in plants: discovering and tailoring genes to unlock the future Fulltext Access 10 Pages 2006
Understanding cellular networks to improve hematopoietic stem cell expansion cultures Fulltext Access 10 Pages 2006
Advances and applications of de novo designed affinity ligands in proteomics Fulltext Access 10 Pages 2006
The effect of RGD fluorosurfactant polymer modification of ePTFE on endothelial cell adhesion, growth, and function Fulltext Access 10 Pages 2006
A tris(2,2′-bipyridyl)cobalt(III)-bovine serum albumin composite membrane for biosensors Fulltext Access 10 Pages 2006
Outgrowth endothelial cells isolated and expanded from human peripheral blood progenitor cells as a potential source of autologous cells for endothelialization of silk fibroin biomaterials Fulltext Access 10 Pages 2006
High efficiency gene transfer using chitosan/DNA nanoparticles with specific combinations of molecular weight and degree of deacetylation Fulltext Access 10 Pages 2006