A cell-based cGMP assay useful for ultra-high-throughput screening and identification of modulators of the nitric oxide/cGMP pathway Fulltext Access 9 Pages 2005
Analysis of protein-surfactant interactions-a titration calorimetric and fluorescence spectroscopic investigation of interactions between Humicola insolens cutinase and an anionic surfactant Fulltext Access 9 Pages 2005
Solution structure of β2-microglobulin and insights into fibrillogenesis Fulltext Access 9 Pages 2005
Characterization of recombinant human protein C inhibitor expressed in Escherichia coli Fulltext Access 9 Pages 2005
Characterization and kinetic analysis of enzyme-substrate recognition by three recombinant lactococcal tripeptidases Fulltext Access 9 Pages 2005
Heterologous expression, purification, crystallization, X-ray analysis and phasing of the acetyl xylan esterase from Bacillus pumilus Fulltext Access 9 Pages 2005
The Saccharomyces cerevisiae ORF YNR064c protein has characteristics of an 'orphaned' epoxide hydrolase Fulltext Access 9 Pages 2005
Comparison of functional properties of mammalian DNA polymerase λ and DNA polymerase β in reactions of DNA synthesis related to DNA repair Fulltext Access 9 Pages 2005
Stereoselective histidine sensor based on molecularly imprinted sol-gel films Fulltext Access 9 Pages 2005
Simultaneous determination of farnesyl and geranylgeranyl pyrophosphate levels in cultured cells Fulltext Access 9 Pages 2005
A live-cell high-throughput screening assay for identification of fatty acid uptake inhibitors Fulltext Access 9 Pages 2005
Semiautomated cell-free conversion of prion protein: Applications for high-throughput screening of potential antiprion drugs Fulltext Access 9 Pages 2005
Cloning and characterization of an Arabidopsis thaliana Nudix hydrolase homologous to the mammalian GFG protein Fulltext Access 9 Pages 2005
A modified cDNA subtraction to identify differentially expressed genes from plants with universal application to other eukaryotes Fulltext Access 9 Pages 2005
Red- and green-emitting firefly luciferase mutants for bioluminescent reporter applications Fulltext Access 9 Pages 2005
Sequence confirmation of synthetic phosphorothioate oligodeoxynucleotides using Sanger sequencing reactions in combination with mass spectrometry Fulltext Access 9 Pages 2005
Gravimetric antigen detection utilizing antibody-modified lipid bilayers Fulltext Access 9 Pages 2005
Quantitative heteroduplex analysis for single nucleotide polymorphism genotyping Fulltext Access 9 Pages 2005
Ferrocene-based Diels-Alder derivatization for the determination of 1α-hydroxyvitamin D3 in rat plasma by high-performance liquid chromatography-electrospray tandem mass spectrometry Fulltext Access 9 Pages 2005
Capillary electrophoresis method for the enzymatic assay of galactosyltransferases with postreaction derivatization Fulltext Access 9 Pages 2005
Study of primary amines for nucleophilic cleavage of cyanylated cystinyl proteins in disulfide mass mapping methodology Fulltext Access 9 Pages 2005
Imaging and spatial distribution of β-amyloid peptide and metal ions in Alzheimer's plaques by laser ablation-inductively coupled plasma-mass spectrometry Fulltext Access 9 Pages 2005
Determination of methotrexate, several pteridines, and creatinine in human urine, previous oxidation with potassium permanganate, using HPLC with photometric and fluorimetric serial detection Fulltext Access 9 Pages 2005
Isolation and mass spectrometry characterization of molecular species of lactosylceramides using liquid chromatography-electrospray ion trap mass spectrometry Fulltext Access 9 Pages 2005
Categorizing rice cultivars based on differences in chemical composition Fulltext Access 9 Pages 2005
Compositional and nutritional properties of selected newly developed lines of cowpea (Vigna unguiculata L.Walp). Fulltext Access 9 Pages 2005
Rapid quantification of iron, copper and zinc in food premixes using energy dispersive X-ray fluorescence Fulltext Access 9 Pages 2005
Antioxidant properties of four edible algae harvested in the Noto Peninsula, Japan Fulltext Access 9 Pages 2005
High-performance liquid chromatographic and spectrofluorometric determination of α-tocopherol in a natural plant: Ferula hermonis (Zalooh root) Fulltext Access 9 Pages 2005
Some physical and chemical properties of ambarella (Spondias cytherea Sonn.) at three different stages of maturity Fulltext Access 9 Pages 2005
9-(2,4-dihydroxyphenyl)-2,3,7-trihydroxyl-6-fluorone as analytical reagent for spectrophotometric determination of molybdenum in plant tissues Fulltext Access 9 Pages 2005
Formation of a misfolded conformation during refolding of HRPA1 in the presence of calcium Fulltext Access 9 Pages 2005
Mutation analysis of the human 5-lipoxygenase C-terminus: Support for a stabilizing C-terminal loop Fulltext Access 9 Pages 2005
Stochastic boundary molecular dynamics simulation of l-ribose in the active site of Actinoplanes missouriensis xylose isomerase and its Val135Asn mutant with improved reaction rate Fulltext Access 9 Pages 2005
An electrochemical investigation of hemoglobin and catalase incorporated in collagen films Fulltext Access 9 Pages 2005
Molecular modeling and chemical modification for finding peptide inhibitor against severe acute respiratory syndrome coronavirus main proteinase Fulltext Access 9 Pages 2005
Exploring the active site of benzaldehyde lyase by modeling and mutagenesis Fulltext Access 9 Pages 2005
Chlamydia pneumoniae AP endonuclease IV could cleave AP sites of double- and single-stranded DNA Fulltext Access 9 Pages 2005
Bacterial expression, folding, purification and characterization of soluble NTPDase5 (CD39L4) ecto-nucleotidase Fulltext Access 9 Pages 2005
Tyrosine B10 and heme-ligand interactions of Lucina pectinata hemoglobin II: control of heme reactivity Fulltext Access 9 Pages 2005
Simultaneous refolding/purification of xylanase with a microwave treated smart polymer Fulltext Access 9 Pages 2005
Proteome analysis of mouse macrophages treated with anthrax lethal toxin Fulltext Access 9 Pages 2005
A novel scheme of dystrophin disruption for the progression of advanced heart failure Fulltext Access 9 Pages 2005
The structure of an enzyme-product complex reveals the critical role of a terminal hydroxide nucleophile in the bacterial phosphotriesterase mechanism Fulltext Access 9 Pages 2005
Calorimetric and spectroscopic investigations of the thermal denaturation of wild type nitrite reductase Fulltext Access 9 Pages 2005
Crystal structure of the complex of the secretory phospholipase A2 from Daboia russelli pulchella with an endogenic indole derivative, 2-carbamoylmethyl-5-propyl-octahydro-indol-7-yl-acetic acid at 1.8 Ã resolution Fulltext Access 9 Pages 2005
Gas chromatography-mass spectrometric analysis of hexanal and heptanal in human blood by headspace single-drop microextraction with droplet derivatization Fulltext Access 9 Pages 2005
Insights into the reaction of β-lactam antibiotics with copper(II) ions in aqueous and micellar media: Kinetic and spectrometric studies Fulltext Access 9 Pages 2005
Homogeneous assays for cellular proteases employing the platinum(II)-coproporphyrin label and time-resolved phosphorescence Fulltext Access 9 Pages 2005
Determining suitable internal standards for mRNA quantification of increasing cancer progression in human breast cells by real-time reverse transcriptase polymerase chain reaction Fulltext Access 9 Pages 2005
Whole-cell-reporter-gene-based biosensing systems on a compact disk microfluidics platform Fulltext Access 9 Pages 2005
Construction of a set Gateway-based destination vectors for high-throughput cloning and expression screening in Escherichia coli Fulltext Access 9 Pages 2005
Development of a lanthanide-based assay for detection of receptor-ligand interactions at the δ-opioid receptor Fulltext Access 9 Pages 2005
Evaluation of the effect of oxygen exposure on human liver microsomal metabolism of mitomycin C in the presence of glutathioneusing liquid chromatography-quadrupole time of flight mass spectrometry Fulltext Access 9 Pages 2005
Simultaneous analysis of prostaglandin glyceryl esters and prostaglandins by electrospray tandem mass spectrometry Fulltext Access 9 Pages 2005
Spectrofluorimetric determination of heparin using a tetracycline-europium probe Fulltext Access 9 Pages 2005
Direct electrochemistry and electrocatalytic activity of catalase incorporated onto multiwall carbon nanotubes-modified glassy carbon electrode Fulltext Access 9 Pages 2005
Metal-catalyzed oxidation reactions and mass spectrometry: The roles of ascorbate and different oxidizing agents in determining Cu-protein-binding sites Fulltext Access 9 Pages 2005
Dual-targeted labeling of proteins using cysteine and selenomethionine residues Fulltext Access 9 Pages 2005
Models of protein modification in Tris-glycine and neutral pH Bis-Tris gels during electrophoresis: Effect of gel pH Fulltext Access 9 Pages 2005
Heterogeneity and function of mammalian MSRs: enzymes for repair, protection and regulation Fulltext Access 9 Pages 2005
Methionine oxidation by reactive oxygen species: reaction mechanisms and relevance to Alzheimer's disease Fulltext Access 9 Pages 2005
Characterization of a thermostable alkaline phosphatase from a novel species Thermus yunnanensis sp. nov. and investigation of its cobalt activation at high temperature Fulltext Access 9 Pages 2005
Targeting protein kinase C and “non-kinase” phorbol ester receptors: Emerging concepts and therapeutic implications Fulltext Access 9 Pages 2005
Coupling phosphoryl transfer and substrate interactions in protein kinases Fulltext Access 9 Pages 2005
Monitoring complex formation in the blood-coagulation cascade using aptamer-coated SAW sensors Fulltext Access 9 Pages 2005
Immunosensor for Mycobacterium tuberculosis on screen-printed carbon electrodes Fulltext Access 9 Pages 2005
Development of a peptide nucleic acid polymerase chain reaction clamping assay for semiquantitative evaluation of genetically modified organism content in food Fulltext Access 9 Pages 2005
Determination of plasma, urine, and bovine serum albumin low-molecular-weight carbonyl levels by capillary gas chromatography with electron-capture and mass-selective detection Fulltext Access 9 Pages 2005
Control of oligonucleotide retention on a pH-stabilized strong anion exchange column Fulltext Access 9 Pages 2005
Development of an oral biosensor for salivary amylase using a monodispersed silver for signal amplification Fulltext Access 9 Pages 2005
Equilibrium studies of a fluorescent tacrolimus binding to surfactant protein A Fulltext Access 9 Pages 2005
A fluorescence-based assay for fatty acid amide hydrolase compatible with high-throughput screening Fulltext Access 9 Pages 2005
FMDV-2A sequence and protein arrangement contribute to functionality of CYP2B1-reporter fusion protein Fulltext Access 9 Pages 2005
Quantification of DNA strand breaks and abasic sites by oxime derivatization and accelerator mass spectrometry: Application to γ-radiation and peroxynitrite Fulltext Access 9 Pages 2005
Quantification of yessotoxin using the fluorescence polarization technique and study of the adequate extraction procedure Fulltext Access 9 Pages 2005
Sensitivity comparison of real-time PCR probe designs on a model DNA plasmid Fulltext Access 9 Pages 2005
Application of a taste evaluation system to the monitoring of Kimchi fermentation Fulltext Access 9 Pages 2005
Bioassay-based screening of microorganisms that degrade dioxin using substrate-immobilized microtubes Fulltext Access 9 Pages 2005
Generation and application of a monoclonal antibody that detects a wide variety of protein tyrosine kinases Fulltext Access 9 Pages 2005
The use of digitonin-permeabilized mammalian cells for measuring enzyme activities in the course of studies on lipid metabolism Fulltext Access 9 Pages 2005
Lipid bilayer tethered inside a nanoporous support: a solid-state nuclear magnetic resonance investigation Fulltext Access 9 Pages 2005
Estimating the number of viable animal cells in multiwell culture-a tetrazolium-based assay Fulltext Access 9 Pages 2005
Comparison of rapid liquid chromatography-electrospray ionization-tandem mass spectrometry methods for determination of glycoalkaloids in transgenic field-grown potatoes Fulltext Access 9 Pages 2005
A cryogenic optical waveguide spectrometer for the measurement of low-temperature absorption spectra of dilute biological samples Fulltext Access 9 Pages 2005
Multiplex polymerase chain reaction for simultaneous quantitation of human nuclear, mitochondrial, and male Y-chromosome DNA: application in human identification Fulltext Access 9 Pages 2005
An amperometric hydrogen peroxide sensor based on immobilization of hemoglobin in poly(o-aminophenol) film at iron-cobalt hexacyanoferrate-modified gold electrode Fulltext Access 9 Pages 2005
Use of multiplex polymerase chain reactions to indicate the accuracy of the annealing temperature of thermal cycling Fulltext Access 9 Pages 2005
Calcium enhances the transfection potency of stabilized plasmid-lipid particles Fulltext Access 9 Pages 2005