Radiochromatographic assay of N-acyl-phosphatidylethanolamine-specific phospholipase D activity Fulltext Access 8 Pages 2005
Recent developments in dynamic electrochemical studies of adsorbed enzymes and their active sites Fulltext Access 8 Pages 2005
A colorimetric determination for glycosidic and bile salt-based detergents: applications in membrane protein research Fulltext Access 8 Pages 2005
Applications of capillary isoelectric focusing with liquid-core waveguide laser-induced fluorescence whole-column imaging detection Fulltext Access 8 Pages 2005
A comparison of different strategies for the construction of amperometric enzyme biosensors using gold nanoparticle-modified electrodes Fulltext Access 8 Pages 2005
Simplex optimization of the variables affecting the micelle-stabilized room temperature phosphorescence of 6-methoxy-2-naphthylacetic acid and its kinetic determination in human urine Fulltext Access 8 Pages 2005
Quantitative analysis of sphingoid base-1-phosphates as bisacetylated derivatives by liquid chromatography-tandem mass spectrometry Fulltext Access 8 Pages 2005
Comparison of full-length versus ligand binding domain constructs in cell-free and cell-based peroxisome proliferator-activated receptor alpha assays Fulltext Access 8 Pages 2005
Tagged polymerase chain reaction subtractive hybridization for the enrichment of phage display random peptide libraries Fulltext Access 8 Pages 2005
Electrochemical study of the interaction between cytochrome c and DNA at a modified gold electrode Fulltext Access 8 Pages 2005
The improvement of sensitivity in lead and cadmium determinations using flame atomic absorption spectrometry Fulltext Access 8 Pages 2005
mRNA and 18S-RNA coapplication-reverse transcription for quantitative gene expression analysis Fulltext Access 8 Pages 2005
Isolation and characterization of subcellular protein fractions from mouse heart Fulltext Access 8 Pages 2005
Microfluidic systems and proteomics: Applications of the electrocapture technology to protein and peptide analysis Fulltext Access 8 Pages 2005
Determination of denaturated proteins and biotoxins by on-line size-exclusion chromatography-digestion-liquid chromatography-electrospray mass spectrometry Fulltext Access 8 Pages 2005
Ion-selective enrichment of tyrosine-sulfated peptides from complex protein digests Fulltext Access 8 Pages 2005
Automated expression and solubility screening of His-tagged proteins in 96-well format Fulltext Access 8 Pages 2005
Stereospecificity of hydride transfer in NAD+-catalyzed 2-deoxy-scyllo-inosose synthase, the key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics Fulltext Access 8 Pages 2005
Expression of Fgf and Tgfβ signaling related genes during embryonic endochondral ossification Fulltext Access 8 Pages 2005
Identification of Dll1 (Delta1) target genes during mouse embryogenesis using differential expression profiling Fulltext Access 8 Pages 2005
LKB1/PAR4 protein is asymmetrically localized in mouse oocytes and associates with meiotic spindle Fulltext Access 8 Pages 2005
Expression of Panza, an α2-macroglobulin, in a restricted dorsal domain of the primitive gut in Xenopus laevis Fulltext Access 8 Pages 2005
Expression of one-hybrid fusions with Staphylococcus aureus lexA in Candida albicans confirms that Nrg1 is a transcriptional repressor and that Gcn4 is a transcriptional activator Fulltext Access 8 Pages 2005
Drifter, a novel, low copy hAT-like transposon in Fusarium oxysporum is activated during starvation Fulltext Access 8 Pages 2005
Development and amplification of multiple co-dominant genetic markers from single spores of arbuscular mycorrhizal fungi by nested multiplex PCR Fulltext Access 8 Pages 2005
A rapid method for promoter exchange in Aspergillus nidulans using recombinant PCR Fulltext Access 8 Pages 2005
The effective molarity of the substrate phosphoryl group in the transition state for yeast OMP decarboxylase Fulltext Access 8 Pages 2005
A theoretical study of the different radical-scavenging activities of catechin, quercetin, and a rationally designed planar catechin Fulltext Access 8 Pages 2005
Protein-protein interactions and cancer: small molecules going in for the kill Fulltext Access 8 Pages 2005
Synthetic approaches towards structurally diverse γ-butyrolactone natural-product-like compounds Fulltext Access 8 Pages 2005
Natural product-like chemical space: search for chemical dissectors of macromolecular interactions Fulltext Access 8 Pages 2005
Protein structure similarity clustering (PSSC) and natural product structure as inspiration sources for drug development and chemical genomics Fulltext Access 8 Pages 2005
High-throughput magnetic resonance imaging in mice for phenotyping and therapeutic evaluation Fulltext Access 8 Pages 2005
Predictive, non-GLP models of secondary pharmacodynamics: putting the best compounds forward Fulltext Access 8 Pages 2005
Recent progress towards the application of hyperthermophiles and their enzymes Fulltext Access 8 Pages 2005
Monitoring the acetohydroxy acid synthase reaction and related carboligations by circular dichroism spectroscopy Fulltext Access 8 Pages 2005
Detection and quantitation of curcumin in mouse lung cell cultures by matrix-assisted laser desorption ionization time of flight mass spectrometry Fulltext Access 8 Pages 2005
Detection of myofibrillar proteins using a step gradient minigel with an ambiguous interface Fulltext Access 8 Pages 2005
Development of a mechanism-based assay for tissue transglutaminase-results of a high-throughput screen and discovery of inhibitors Fulltext Access 8 Pages 2005
Activity assay of mammalian 2-cys peroxiredoxins using yeast thioredoxin reductase system Fulltext Access 8 Pages 2005
Discrimination between closed and open forms of lipases using electrophoretic techniques Fulltext Access 8 Pages 2005
A fluorescence resonance energy transfer-based probe to monitor nucleosome structure Fulltext Access 8 Pages 2005
Evaluation of alkoxyresorufins as fluorescent substrates for cytochrome P450 BM3 and site-directed mutants Fulltext Access 8 Pages 2005
Rapid simultaneous determination of metabolic clearance of multiple compounds catalyzed in vitro by recombinant human UDP-glucuronosyltransferases Fulltext Access 8 Pages 2005
Intracellular monitoring of superoxide dismutase expression in an Escherichia coli fed-batch cultivation using on-line disruption with at-line surface plasmon resonance detection Fulltext Access 8 Pages 2005
Biochemical and cell-based assays for characterization of BACE-1 inhibitors Fulltext Access 8 Pages 2005
Real-time polymerase chain reaction-based exponential sample amplification for microarray gene expression profiling Fulltext Access 8 Pages 2005
A continuous spectrophotometric assay for human cystathionine beta-synthase Fulltext Access 8 Pages 2005
Conformational impurity of disulfide proteins: Detection, quantification, and properties Fulltext Access 8 Pages 2005
Application of high-performance liquid chromatography-electrospray ionization-mass spectrometry to measure microsomal membrane transport of glucuronides Fulltext Access 8 Pages 2005
Synthesis and characterization of azole isoflavone inhibitors of aromatase Fulltext Access 8 Pages 2005
Napthalimidobenzamide DB-51630: a novel DNA binding agent inducing p300 gene expression and exerting a potent anti-cancer activity Fulltext Access 8 Pages 2005
Intrastrand base-stacking buttresses widening of major groove in interstrand cross-linked B-DNA Fulltext Access 8 Pages 2005
Understanding human 15-hydroxyprostaglandin dehydrogenase binding with NAD+ and PGE2 by homology modeling, docking and molecular dynamics simulation Fulltext Access 8 Pages 2005
New ether diglycosides from Matayba guianensis with antiplasmodial activity Fulltext Access 8 Pages 2005
Chemical regulation of abscisic acid catabolism in plants by cytochrome P450 inhibitors Fulltext Access 8 Pages 2005
The preparation of HEK293 α1A or HEK293 α1B cell membrane stationary phase and the chromatographic affinity study of ligands of α1 adrenoceptor Fulltext Access 8 Pages 2005
DigiTag assay for multiplex single nucleotide polymorphism typing with high success rate Fulltext Access 8 Pages 2005
A sensitive immunoassay based on electropolymerized films by capacitance measurements for direct detection of immunospecies Fulltext Access 8 Pages 2005
Measurement of steroid synthesis in zona glomerulosa cells by liquid chromatography-electrospray ionization-mass spectrometry: inhibition by nitric oxide Fulltext Access 8 Pages 2005
Continuous-flow/stopped-flow system using an immunobiosensor for quantification of human serum IgG antibodies to Helicobacter pylori Fulltext Access 8 Pages 2005
Optical zymography for specific detection of urokinase plasminogen activator activity in biological samples Fulltext Access 8 Pages 2005
A novel means of asymmetric linker attachment to DNA molecule by RecA-mediated multistranded DNA formation Fulltext Access 8 Pages 2005
Analytical method for ubiquinone-9 and ubiquinone-10 in rat tissues by liquid chromatography/turbo ion spray tandem mass spectrometry with 1-alkylamine as an additive to the mobile phase Fulltext Access 8 Pages 2005
Tandem mass spectrometric assay of succinylacetone in urine for the diagnosis of hepatorenal tyrosinemia Fulltext Access 8 Pages 2005
Rapid sodium cyanide depletion in cell culture media: Outgassing of hydrogen cyanide at physiological pH Fulltext Access 8 Pages 2005
Alkaline phosphatase vs luciferase as secreted reporter molecules in vivo Fulltext Access 8 Pages 2005
A new polyamine derivative, a structural analog of spermine, with in vivo activity as an inhibitor of ethanol appetite Fulltext Access 8 Pages 2005
Fluorescence imaging of the activity of glucose oxidase using a hydrogen-peroxide-sensitive europium probe Fulltext Access 8 Pages 2005
Time-resolved Forster resonance energy transfer assays for the binding of nucleotide and protein substrates to p38α protein kinase Fulltext Access 8 Pages 2005
Analysis of hyaluronan content in chondroitin sulfate preparations by using selective enzymatic digestion and electrospray ionization mass spectrometry Fulltext Access 8 Pages 2005
A spectrophotometric method for the quantification of an enzyme activity producing 4-substituted phenols: Determination of toluene-4-monooxygenase activity Fulltext Access 8 Pages 2005
Immobilization and hybridization of oligonucleotides on maleimido-terminated self-assembled monolayers Fulltext Access 8 Pages 2005
An ultrasensitive fluorescent assay for the in vivo quantification of superoxide radical in organisms Fulltext Access 8 Pages 2005
Synthesis and study of the electrophoretic behavior of mannan conjugates with cyclic peptide analogue of myelin basic protein using lysine-glycine linker Fulltext Access 8 Pages 2005
pH determination by pyranine: Medium-related artifacts and their correction Fulltext Access 8 Pages 2005
Paclitaxel quantification in mouse plasma and tissues containing liposome-entrapped paclitaxel by liquid chromatography-tandem mass spectrometry: application to a pharmacokinetics study Fulltext Access 8 Pages 2005
Quantitative analysis of the microbial metabolome by isotope dilution mass spectrometry using uniformly 13C-labeled cell extracts as internal standards Fulltext Access 8 Pages 2005
New methods for measuring macromolecular interactions in solution via static light scattering: basic methodology and application to nonassociating and self-associating proteins Fulltext Access 8 Pages 2005