کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10157968 | 1666499 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Design, display and immunogenicity of HIV1 gp120 fragment immunogens on virus-like particles
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کلمات کلیدی
CD4BSPBSPbSbPBSTVLPsSMCCCysENVRLUCD4 binding siteenzyme-linked immune sorbent assay - آزمون آنزیم وابسته به ایمنی بدنNeutralizing antibodies - آنتی بادیهای ناتریالELISA - تست الیزاSPR - تشدید پلاسمون سطحیSurface plasmon resonance - تشدید پلاسمون سطحیvirus-like particles - ذرات ویروس مانندCysteine - سیستئینphosphate buffer saline - فسفات بافر شورNanoparticles - نانوذراتrelative luminescence units - واحد لومینسانس نسبیresponse units - واحدهای پاسخVaccine - واکسنProtein stability - پایداری پروتئینcapsid protein - پروتئین کپسیدEnvelope glycoprotein - گلیکوپروتئین پاکت
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The broadly neutralizing antibody against HIV-1, b12, binds to the CD4 binding site (CD4bs) on the outer domain (OD) of the gp120 subunit of HIV-1 Env. We have previously reported the design of an E. coli expressed fragment of HIV-1 gp120, b122a, containing about 70% of the b12 epitope with the idea of focusing the immune response to this structure. Since the b122a structure was found to be only partially folded, as assessed by circular dichroism and protease resistance, we attempted to stabilize it by the introduction of additional disulfide bonds. One such mutant, b122a1-b showed increased stability and bound b12 with 30-fold greater affinity as compared to b122a. Various b122a and OD fragment proteins were displayed on the surface of Qβ virus-like particles. Sera raised against these particles in six-month long rabbit immunization studies could neutralize Tier1 viruses across different subtypes with the best results observed with b122a1-b displayed particles. Significantly higher amounts of antibodies directed towards the CD4bs were also elicited by particles displaying b122a1-b. This study highlights the ability of fragment immunogens to focus the antibody response to the conserved CD4bs of HIV-1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 36, Issue 42, 8 October 2018, Pages 6345-6353
Journal: Vaccine - Volume 36, Issue 42, 8 October 2018, Pages 6345-6353
نویسندگان
Mansi Purwar, Jonathan K. Pokorski, Pranveer Singh, Sanchari Bhattacharyya, Heather Arendt, Joanne DeStefano, Celia C. La Branche, David C. Montefiori, M.G. Finn, Raghavan Varadarajan,