Connexin multi-site phosphorylation: Mass spectrometry-based proteomics fills the gap

► We provide an up to date overview of mass spectrometry (MS)-based proteomics. ► Cx43 appears to undergo multiple levels of multisite phosphorylation. ► Bioinformatics reveal the majority of sites are consensus to multiple kinases. ► Multisite phosphorylation sequence and order provides insight into the Cx code.