Probing membrane permeabilization by the antibiotic lipopeptaibol trichogin GA IV in a tethered bilayer lipid membrane

► Trichogin GA IV forms ion channels in lipid bilayers following a kinetics of nucleation and growth. ► Trichogin GA IV ion channels exhibit a voltage-gated behavior in lipid bilayers. ► Trichogin GA IV ion channels permeabilizes membranes at trans-negative transmembrane potentials of − 80 ÷ − 90 mV.