Bafibrinase: A non-toxic, non-hemorrhagic, direct-acting fibrinolytic serine protease from Bacillus sp. strain AS-S20-I exhibits in vivo anticoagulant activity and thrombolytic potency

► A 32.3 kDa fibrinolytic serine protease was purified from Bacillus sp. AS-S20-I. ► Novelty of the purified protease was deduced from its peptide mass fingerprinting. ► Protease showed better thrombolytic and anticoagulant properties than human plasmin. ► Protease was found as non-toxic and non-hemorrhagic in in vitro and in animal model. ► Our study suggested application of this protease as a better thrombolytic drug.