Molecular cloning and characterization of antimicrobial peptides from skin of the broad-folded frog, Hylarana latouchii

► 7 AMP precursors were cloned from skin of Hylarana latouchii. ► Their actual presence was confirmed by RP-HPLC and LC-MS/MS proteomics approach. ► 4 different ways were used to prepare the sample. ► Incompletely digestion and GPF analysis were used to achieve higher proteome coverage.