Regulation of autophagosome formation by Rho kinase

► Elevated Rho kinase function inhibits autophagy. ► Inhibition of Rho kinase enhances starvation-induced autophagic degradative flux. ► Enlarged autophagosomal compartments are formed when Rho kinase is inhibited. ► Enlarged autophagosomes are enriched for the autophagy regulatory kinase ULK1.