Role of nitric oxide produced by iNOS through NF-κB pathway in migration of cerebellar granule neurons induced by Lipopolysaccharide

LPS increased NO
- - production, iNOS protein levels and NF-κB nuclear levels; concomitantly with NO
- - production, LPS increased the neuronal migration as compared to non stimulated cultures. The necessary roles of the NO
- - and iNOS were demonstrated by chelating of NO
- - with hemoglobin and the inhibition of iNOS by 1400 W. Each of these treatments reduced neuronal migration induced by LPS. The role of NF-κB was showed by using the inhibitor JSH-23, which decreased NO
- - production and neuronal migration in LPS activated cultures. These results suggest that neuronal migration during development is susceptible to be modified by pro-inflammatory stimulus such as LPS through intracellular pathways associated with their receptors.