کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10816150 | 1058543 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Involvement of connexin43 in the EGF/EGFR signalling during self-renewal and differentiation of neural progenitor cells
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کلمات کلیدی
EGFRGJICFRAPbFGFGFAPEGF5-ethynyl-2′-deoxyuridine - 5-ethynyl-2'-deoxyuridineEdU - EDUSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNAgap junctions - اتصالات شکاف دارgap junctional intercellular communication - ارتباط بین سلولی بین دو سلولیDifferentiation - تفکیکepidermal growth factor - عامل رشد اپیدرمیGrowth factor - فاکتور رشدbasic fibroblast growth factor - فاکتور رشد فیبروبلاست پایهfluorescence recovery after photobleaching - فلوئورسانس پس از فوتوبلاسیکGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالconnexin - کنسکسینEpidermal growth factor receptor - گیرنده فاکتور رشد اپیدرمال
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Neural progenitor cells (NPCs) are sensitive to epidermal growth factor (EGF), which is essential for their self-renewal. Recently we showed that high level of connexin43 (Cx43) expression and gap junctional intercellular communication (GJIC) are also required to maintain NPCs in a proliferative state. In this study the connection between EGF/EGFR signalling and Cx43 expression was investigated during proliferation and differentiation of cultured ReNcell VM197 human NPCs. We found that EGF, but not basic fibroblast growth factor (bFGF), strongly stimulated both Cx43 expression and GJIC in proliferating cells. This stimulatory effect was blocked by AG1478, a specific inhibitor for EGFR kinase. Notably, knockdown of Cx43 strongly inhibited the cell proliferation promoted by EGF/EGFR signalling. High sensitivity to EGF was still maintained in differentiated NPCs. Administration of EGF to differentiating cells led to a pronounced increase (9-fold) of Cx43 expression and a re-induction of proliferation. This strong impact of EGF was found to correlate with a surprisingly massive 60-fold up-regulation of EGFR expression in differentiated cells. Our data argue for a mutual regulation between Cx43 expression and EGF/EGFR signalling during self-renewal and differentiation of NPCs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 25, Issue 12, December 2013, Pages 2676-2684
Journal: Cellular Signalling - Volume 25, Issue 12, December 2013, Pages 2676-2684
نویسندگان
Heiko Lemcke, Sergei A. Kuznetsov,